Fig. 4

Prediction and Confirmation of novel miRNAs in P. clarkia. a Secondary structure of novel pre-miRNA in P. clarkia. b The expression levels of the novel miRNAs in P. clarkia detected by semi-quantitative RT-PCR with 30 cycles. c The expression levels of the novel pre-miRNAs in neuro, gill, muscle and heart in P. clarkia